Overlap PCR is used to create gene knockout constructs. The method of Szewczyk et al can be used with primer sets for pyrG and ptrA. The primer sets were developed by Brian Mack at USDA.
Payne Lab
Overlap PCR is used to create gene knockout constructs. The method of Szewczyk et al can be used with primer sets for pyrG and ptrA. The primer sets were developed by Brian Mack at USDA.